Rhamnolipids  biosurfactant

Definition: Rhamnolipids are anionic biosurfactants produced by *Pseudomonas aeruginosa* through microbial fermentation of soybean 

oil under aerobic conditions. Composed of sugar rings and fatty acid functional groups, they belong to the glycopeptide lipid class, with a 

molecular weight of 200-500D, and are easily absorbed, decomposed, and utilized by crops.

Function :

(1) Immune Stimulation: Rhamnolipids are glycolipids that activate plant immunity, enhance disease resistance, and improve stress 

tolerance. They can induce the production of resistance proteins in crops, simultaneously induce lignin formation, promote wound 

healing, and prevent pathogen invasion. They can also induce the expression of plant resistance-related genes, leading to disease 

resistance responses in crops, enhancing their active immunity, and preventing premature aging. 

(2) Fertilizer Enhancement: Rhamnollipids possess an amphiphilic structure. The hydrophobic groups adsorb onto the waxy surface,

while the hydrophilic groups extend into the fertilizer solution, forming a directional adsorption film. This reduces the surface tension

and contact angle of the liquid, allowing it to spread, extend, and penetrate, thus promoting the absorption of nutrients from the fertilizer.

As a glycopeptide lipid, it can strongly activate soil microbial activity, promoting the reproduction and expansion of beneficial microbial 

communities.

(3) Pesticide Enhancement: As a surfactant with a relatively large molecular weight, rhamnollipids possess excellent dispersibility and 

adsorption capacity due to their structural characteristics. This allows pesticides to adhere better to the surface during use, preventing 

them from easily detaching or migrating, ensuring the long-term stability of pesticide effects. Furthermore, its surface activity promotes 

the penetration of pesticides into plants, achieving the purpose of solubilization and enhanced efficacy. 

(4) Soil Improvement: Rhamnollipin promotes soil aggregate formation, breaks up soil compaction, loosens soil, enhances microbial 

activity, replenishes soil fertility, promotes nutrient absorption and utilization, and promotes root and seedling growth.

(5) Chelating Micronutrients: Rhamnollipin biosurfactants have the function of chelating some micronutrients. In a weakly alkaline 

environment, the carboxyl groups ionized can fix micronutrients around the roots, reducing the possibility of rapid loss of micronutrients 

in the soil, promoting the absorption and utilization of micronutrients by crops, and ensuring the long-lasting effect of fertilizer.

(6) Antibacterial and Insect-Repellent Effects: The lipophilic groups of rhamnollipin can dissolve the fatty walls on the surface of bacteria, 

destroy the semi-permeable membrane, and cause bacterial death. When mixed with other fungicides, it can effectively improve the 

insecticidal effect.